The airline's online and telephone booking system went live on Wednesday.
Kiwi Regional Airlines will start scheduled flights on October 27, subject to regulatory approval, servicing Dunedin, Queenstown, Nelson and Hamilton.
According to its website, a direct flight from Nelson to Dunedin return on October 28 will cost $194 each way.
Nelson to Hamilton will cost $184, while the return flight is listed at $139.
Nelson to Queenstown is $134 and Queenstown to Nelson is $194.
Prices are subject to change but provide an early insight into what customers might expect to pay for the new service, which aims to fill gaps in the domestic flight market.
"Fares on Kiwi flights will be completely transparent, with no unexpected add-ons," chief executive Ewan Wilson said.
"What you see on your computer or phone screen will be what you are charged.
Mr Wilson said children, aged two to 15, will have seats booked at 75 per cent of the applicable fare so long as they are accompanied by a paying adult.
This is a much more family-friendly policy than travellers will have seen recently," he said.
"We are the only ones offering a child discount, and the only ones extending the child age until they turn 16."